Ponnuswamy Karkuzhali

Department of Pathology, Sree Balaji Medical College & Hospital Affiliated to Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India


  • Research   
    Role of Immunohistochemistry Versus Hematoxylin and Eosin and Special Stains in Helicobacter Pylori Detection in Gastric Lesions
    Author(s): Ayushi Narain and Ponnuswamy Karkuzhali*

    Helicobacter pylori can be demonstrated in tissue by Gram stain, Giemsa stain, haematoxylin & eosin stain, Warthin-Starry silver stain, acridine orange stain, and phase-contrast microscopy. Hence the present study was premeditated to analyse the role of Immunohistochemistry versus Hematoxylin and Eosin and special stains in detection of Helicobacter pylori in gastric lesions. A total of 455 gastric biopsy specimens were received over a period of 2 years from October 2015 to September 2017. Of these 100 cases were selected which had adequate data. 60 cases were randomly selected and analysed for Helicobacter pylori infection using H&E, Giemsa and IHC. The most associated endoscopic finding with gastritis in the present study was erosions followed by erythema (linear bands/erythematous mucosa). Sensitivity and specificity of Giemsa was 95.2 % and 100% .. Read More»

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