The Effect of Adding Poloxamer Surfactant on the Penetration Depth of NaOCl and NaOH into Dentinal Tubules
Objectives: The purpose of this in vitro study was to compare the effect of adding poloxamer surfactant to irrigant solutions on the penetration depth inside dentinal tubules.
Materials and method: 63 human single roots of permanent premolar (7 for each group) were used in this in vitro study. Roots embedded in container (filled with impression silicon) were instrumented with ProTaper Rotary instruments till size F4, each group (7 roots) were irrigated with one of the following solution: NaOH (5% (A1), 2.5/(A2), 0.5%(A3)), NaOH+Poloxamer (5%(B1), 2.5%(B2), 0.5%(B3)), NaOCl 5.25%(C1), NaOCl+Poloxamer (C2), and Normal Saline(D). Each group was irrigated with 3 ml of EDTA 17% for 1 Minute to remove smear layer then washing with normal saline. After that, the roots were filled with methylene blue that washed later by one of solutions used in this study. By longitudinal sectioning of the roots the specimens might be ready for imaging and examining with digital microscope, the differences in color for each root images were measured with Photoshop program. Then the result analyzed statistically with Anova test, Post hock, and T test.
Results: The study revealed that NaOH showed highest penetration than that of NaOCl, and the adding of Poloxamer surfactant significantly increase the penetration inside dentinal tubules for both solutions. There was also a significant difference in penetration depth between root thirds.
Conclusion: Adding poloxamer surfactant significantly increased penetration depth of irrigant solutions inside dentinal tubule, and there was a significant difference in penetration among solutions and between root thirds.