A.J. Manjula Devi
Department of Biochemistry, Sree Balaji Medical College & Hospital Affiliated to Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, IndiaPublications
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Research Article
Attempts to Develop a Recombinant Protein with Antioxidant and Thrombol Ytic Activity - In-Vitro Study
Author(s): C.Revathy and A.J. Manjula Devi*
The objectives were framed bearing in mind to generate a synergetic protein of higher efficacy so as to handle both the thrombotic status and ischemia mediated free radical injury to fight Myocardial infarction. Amplification of human Thioredoxin gene from lung cancer cell line A549.Cloning of human Streptokinase in pRSET A at Barn HI and Eco RI site and fusion of human thioredoxin in the recombinant clone pRSET A/Sk at Ndel and Barn HI site In this study, the human thioredoxin isolated from lung cancer cell lines is fused with streptokinase to generate a fusion construct so as to develop a synergetic protein of higher efficacy in the thrombolytic and the antioxidant activity. Expression studies and solubility of the recombinant protein was studied., Maximum soluble fraction achieved by optimizing various parameters like host strain, optical density, post induction temperature, inducer c.. Read More»