Bedri Selim Benek

Department of Physiology, Medical Faculty, Abant Izzet Baysal University, Bolu, Turkey


  • Research   
    Protective Effects of Trimetazidine Against Bilirubin-Induced Neurotoxicity
    Author(s): Bedri Selim Benek, Recep Bayram, Ummugulsum Benek and Kenan Gumustekin*

    Background and aims: We aimed to research the effects of limiting oxidation and blocking membrane calcium channels on induced-bilirubin neurotoxicity. For this purpose, we used anti-ischemic drugs that Trimetazidine (TMZ) has features as limiting membrane damage induced by reactive oxygen radicals and decreasing overload calcium into the cell. Methods: In this study, the SH-SY5Y neuroblastoma cell line was used. Cells at a concentration of 3x104 cell/100μl were seeded to a 96-well plate. The toxic doses of bilirubin and the protecting effect of TMZ were determined by the MTT test. To detect the poisonous mechanism of bilirubin, the Muse Cell Analyzer Annexin V&Dead Cell assay kit protocol was applied. Determination of the intracellular free oxygen radicals was made with the flow cytometric oxidative stress assay according.. Read More»

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