Paikrao VM

Department of Anatomy, NKP Salve Institute of Medical Sciences and Research Centre and Lata Mangeshkar Hospital, Digdoh Hills, Hingna Road, Nagpur, India


  • Research   
    Study of Genotoxic Effects of Artificially Induced UV-Radiation on Human Lens Epithelial Cell (HLEC) Culture by Using Dye Exclusion Assay
    Author(s): Huddar MD and Paikrao VM*

    Cataract is one of major cause of blindness, 44% cases of blindness is due to cataract only. In developing countries such as India cataract is more common, and it manifest earlier in life than the most developed countries of the world. Cataract is more common in dry and hot area where sun (UV) exposure is more than prolong cloudy cover. In this study we are aimed to compare effects of UV-ray irradiation on cell viability of HLEC (Human lens epithelial cell) culture by using trypan blue dye exclusion assay against the unexposed control HLEC culture. Total 20 cataract patients from Dept. of Ophthalmology of Lata Mangeshkar Hospital, Nagpur were included in this study. The capsulorhexis sample from cataract surgery taken for study from eye OT. The capsulorhexis sample without any UV-ray exposure taken as control. For culture of HLEC’s RPMI 1640 medium containing 1.. Read More»

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