Reza Aghabozorgi
Khansari Hospital and Department of Internal Medicine, School of Medicine, Arak University of Medical Sciences, Arak, IranPublications
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Research
Detection of CHEK2 Ile157Thr mutation in cancer patients by using allele specific PCR
Author(s): Aliarash Anoushirvani, Azam Ahmadi*, Mohammad Arjomandzadegan*, Reza Aghabozorgi, Mehrana Jafari and Farnaz Mehrabbeygi
Aims: The checkpoint kinase 2 (CHEK2) was identified as a gene in cell cycle control upon DNA damage. The mutation in this gene is associated with some kinds of cancer including breast and lung tumors. The aim of this study was to investigate, I157T missense mutation of CHEK2 gene by Allele specific PCR (ASP) technique in clinical samples of breast and non-small cell lung cancer (NSCLC) samples. Method: In this study, we investigated I157T mutation of CHEK2 gene in whole blood DNA from 30 breast cancers (BCs), 20 NSCLC patients and 30 control samples. The primers of ASP were designed to detect non-mutant status at the position of 430 CHEK2. The data were analyzed statistically. Results: The results of the amplification reaction in used samples were indicated the accuracy of designed primers. The results of this stu.. Read More»