Investigation of TNF-?, LT-? and TNFR-2 gene Polymorphisms in Patients with Periodontitis in Turkey
Introduction: One of the cytokines involved in periodontal breakdown in periodontitis is; Tumor necrosis factor (TNF), with two types; tumor necrosis factor-α (TNF-α) and lymphotoxin-α (LT-α). Aim: In the present study, the individual frequency of TNF-α -308 promoter, LT-α +252 intron, and TNF receptor-2 (TNFR-2) +587 exon polymorphisms associated with chronic (CP) and generalized aggressive periodontitis (GAP) were evaluated in Turkish population. Materials and Methods: This research was conducted on 224 periodontitis patients (P) (119 CP, 105 GAP patients) and 85 healthy controls (C). Blood samples were collected, and specific DNA regions were amplified by PCR method. Investigated gene polymorphisms were determined by RFLP (Restriction Fragment Length Polymorphism). Statistical analyses, including linkage analyses were performed. Results: While there was no statistically significant difference in LT-α +252 polymorphisms between the patients and controls, significant associations between TNF-α -308 GA genotype and CP and GAP were determined. For TNFR-2 (+587) T/G polymorphism; TT, TG and GG genotype and allele frequencies were analysed and significant differences were found in genotype and allele frequencies between the control and CP and GAP groups (p<0,001). Conclusion: This is the first study investigating the TNF-α, LT-α and TNFR-2 polymorphisms in CP and GAP in Turkish population. Significant differences were found in genotype and allele frequencies, in CP and GAP groups.